Programme of the day
10.00 Welcome, introduction to CheckNano – Jacek Fiutowski, SDU
10.45 Toxicology of silver nanoparticles, Frank Kjeldsen, SDU Odense
11:15 Discussion and questions
11.30 Discussion on sample preparation and pretreatment – led by Antje Labes, Hochschule Flensburg
13.00 Effects of nanoparticles in the human body – crossing of biological barriers, Yvonne Kohl, Fraunhofer IBMT
13.30 Discussion round: Perspectives
14.30 Tour of SDU Sønderborg
15.30 End of the day
Ms Yvonne Kohl will be accompanied by one of her colleagues, Thorsten Knoll who is also working in the field and will enrich the discussion.
Ivonne Kohl, Dr. Group manager, Fraunhofer Institute for Biomedical Engineering IBMT, Sulzbach, Germany
Title: Effects of nanoparticles in the human body – crossing of biological barriers
Positive effects of nanoparticles (NPs) go hand in hand with risks for humans and the environment, which are unfortunately still unknown these days. NPs can enter the human organism via inhalation, oral uptake or dermal contact. After overcoming the external barriers, the NPs enter the blood system, circulate in the organism and can accumulate in the organs and/or trigger toxic effects.
A serious lack of information concerning the potential health risks of nano-sized materials leads to the need for new sensitive models and technologies to assess the risks of nanomaterial-cell interactions in vitro. In this talk we introduce the development and application of advanced in vivo-like non-animal models and new screening tools for nanomaterial profiling and non-invasive cell characterization. We discuss disadvantages of standard toxicity assays for nanosafety screening and present optimized solutions. NP-induced effects in 2D and 3D cultures, in non-cancer and cancer cells, as well as the translocation of NPs in human in vitro co-culture models will be discussed. Furthermore, we present results of the H2020 project HISENTS “High level Integrated Sensor for Nanotoxicity Screening“ (GA no. 685817), like chip-based microfluidic modules for in vitro studies, and discuss future perspectives.
Frank Kjeldsen, Professor, Department of Biochemistry and Molecular Biology, SDU Odense
Title: Toxicology of silver nanoparticles
The importance of future sustainable nanotechnology justifies development of improved analysis methods to assess the interactions of nanoparticles with cells from living organisms. In recent years, proteomics has emerged as a powerful informative technique to infer the cellular biological impact of nanoparticle exposures. In this presentation I will demonstrate the proteomics workflow of our laboratory and give examples of biological conclusions drawn from such studies. Similarly, we have also found proteomics to be very applicable for the characterization of protein corona. Admitted, proteomics analyses are labor-intensive, which limits for now the high-through-put screening of nano-bio interactions. The solution could be a carefully optimized proteomics platform, which we have ambitions to create.